Isolation and characterization of 11 tetranucleotide microsatellite loci in the Egyptian mongoose (Herpestes ichneumon)
MÓNICA RODRIGUES*§, CARLOS A. FERNANDES*†§, FRANCISCO PALOMARES‡, ISABEL R. AMORIM¶¥, MICHAEL W. BRUFORD† and MARGARIDA SANTOS-REIS*
*Centro de Biologia Ambiental, Universidade de Lisboa, Campo Grande Edifício C2 5° Piso, 1749 016 Lisboa, Portugal, †Cardiff University, Museum Avenue, Cardiff CF10 3US, UK, ‡Departamento de Biología de la Conservación, Estación Biológica de Doñana-CSIC, Avenida de Maria Luisa s/n, Pabellón del Perú, E-41013 Seville, Spain, ¶Departamento de Ciências Agrárias-CITAA, Universidade dos Açores, 9700-851 Angra do Heroísmo, Portugal, ¥Grupo Lobo, 1749-016 Lisboa, Portugal
We report the isolation of 11 polymorphic tetranucleotide microsatellite loci in the Egyptian mongoose (Herpestes ichneumon). In a sample of 27 individuals, we observed between 4 and 7 alleles per locus and their observed and expected heterozygosities ranged from 0.37 to 0.85 and from 0.44 to 0.79, respectively. All genotypic frequencies conformed to Hardy–Weinberg equilibrium expectations and there were no instances of linkage disequilibrium detected between pairs of loci.
*Centro de Biologia Ambiental, Universidade de Lisboa, Campo Grande Edifício C2 5° Piso, 1749 016 Lisboa, Portugal, †Cardiff University, Museum Avenue, Cardiff CF10 3US, UK, ‡Departamento de Biología de la Conservación, Estación Biológica de Doñana-CSIC, Avenida de Maria Luisa s/n, Pabellón del Perú, E-41013 Seville, Spain, ¶Departamento de Ciências Agrárias-CITAA, Universidade dos Açores, 9700-851 Angra do Heroísmo, Portugal, ¥Grupo Lobo, 1749-016 Lisboa, Portugal
We report the isolation of 11 polymorphic tetranucleotide microsatellite loci in the Egyptian mongoose (Herpestes ichneumon). In a sample of 27 individuals, we observed between 4 and 7 alleles per locus and their observed and expected heterozygosities ranged from 0.37 to 0.85 and from 0.44 to 0.79, respectively. All genotypic frequencies conformed to Hardy–Weinberg equilibrium expectations and there were no instances of linkage disequilibrium detected between pairs of loci.
(In Molecular Ecology Resources)
Etiquetas: Biodiversidade, genética, publicações
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